The recommended order of draw for plastic collection tubes is:
- First – blood culture bottle or tube (yellow or yellow-black top)
- Second – coagulation tube (light blue top).
- Third – non-additive tube (red top)
- Last draw – additive tubes in this order:
What test goes in what color tube?
Blood Collection Tubes
| Tube cap color | Additive | Common laboratory tests |
|---|---|---|
| Lavender or pink | Potassium EDTA | Hematology and blood bank |
| Gray | Sodium fluoride, and sodium or potassium oxalate | Glucose (especially when testing will be delayed), blood alcohol, lactic acid |
What is each tube used for in phlebotomy?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | USES/COMMENTS |
|---|---|---|
| Green | Sodium heparin(100 USP Units) | Ammonia, Lactate, HLA Typing |
| Tan | K2 EDTA | Lead levels |
| Yellow | ACD Solution A consists of trisodium citrate, citric acid and dextrose | DNA Studies, HIV Cultures |
| Pink | (K2)EDTA | Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load |
What are the 3 veins used for venipuncture?
The most site for venipuncture is the antecubital fossa located in the anterior elbow at the fold. This area houses three veins: the cephalic, median cubital, and basilic veins (Figure 1).
Which tube do you draw first?
EDTA tubes
The order of draw is based on CLSI Procedures and Devices for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
What is order of draw on tubes?
The draw order for specimen tubes is as follows:
- Blood culture.
- Blue tube for coagulation (Sodium Citrate)
- Red No Gel.
- Gold SST (Plain tube w/gel and clot activator additive)
- Green and Dark Green (Heparin, with and without gel)
- Lavender (EDTA)
- Pink – Blood Bank (EDTA)
- Gray (Oxalate/Fluoride)
What is heparin tube?
VACUETTE® heparin tubes are used in determinations for clinical chemistry and immunology, hormones, TDM and microbiological serology. Heparin tubes from Greiner Bio-One are available in three different versions: LH Lithium Heparin, LH Lithium Heparin Sep and NH Sodium Heparin.
What color tube is CBC?
The following tests may be drawn in the same LAVENDER (PURPLE) top tube: CBC, SED RATE (ESR), RETICULOCYTE Draw an extra LAVENDER for each (GLYCOHEMOGLOBIN and BNP). 9. GLUCOSE is always drawn in a GRAY top tube. No additional test may be drawn with this tube.
What is the yellow tube for in phlebotomy?
Yellow-top tube (ACD) This tube contains ACD, which is used for the collection of whole blood for special tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
What color tube is CMP?
Green Tube/Plasma: Centrifuge after collection. Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
What is antecubital fossa?
Introduction. The cubital fossa is an area of transition between the anatomical arm and the forearm. It is located in a depression on the anterior surface of the elbow joint. It is also called the antecubital fossa because it lies anteriorly to the elbow (Latin cubitus) when in standard anatomical position.
What are the 5 sites for venipuncture?
What is Antecubital Fossa?
- Median cubital vein.
- Cephalic vein.
- Basilic vein.
What are the materials needed for venipuncture?
Blood Collection Process: Venipuncture. Venipuncture Procedure. ROUTINE VENIPUNCTURE GUIDELINES. MATERIALS. 1. Safety Needles, 22g or less. 2. Butterfly needles. 21g or less. 3. Syringes. 4. Vacutainer tube holder. 5. Transfer Device 6. Blood Collection Tubes. The vacuum tubes are designed to draw a predetermined volume of blood.
What should you do if you have a difficult venipuncture?
If the blood is flowing wall of the vein. Loosen the tourniquet, as it may be obstructing blood flow. If you are vacutaining, try another tube – there may be no vacuum in the tube. process with a new site. In the case of a difficult venipuncture, an someone else try.
How to use a winged blood collection set for venipuncture?
NOTE: When using a winged blood collection set for venipuncture and a coagulation tube is the first tube needed, first draw a discard tube (plain red top or light blue top). The discard tube does not need to be filled completely. 1. Assemble the necessary equipment appropriate to the patient’s physical characteristics.
What is the correct order of draw for a venipuncture?
This order of draw should be followed whenever multiple tubes are drawn during a single venipuncture. This is to prevent cross contamination by the tube additives that could lead to erroneous results. 1. Blood Culture 2. Light Blue Top (plasma): 3.2% sodium citrate.
